Disinfectant Efficacy Study

In-vitro Study (in Suspension):

• Ready-to-Use solutions of each antimicrobial product are challenged with each organism in duplicate or triplicate test tubes. After a determined dwell period survivors are extracted and recovered for determination of a log₁₀ reduction value.
• The extraction method is validated for its neutralization efficacy against all solutions and organisms.

In-situ Study (with Surfaces):

• Surface coupons are cleaned and sterilized to eliminate any possible background contamination.
• The extraction method for each organism from each surface type is qualified demonstrating an acceptable efficiency.
• The recovery and incubation method is validated for its neutralization efficacy using an un-inoculated coupon.  This is conducted for each organism and surface coupon combination.
• Duplicate or triplicate coupons are inoculated with an organism challenge of not less than 10⁵ CFU.  A coupon set is prepared, disinfected, and allowed to dwell for a period of time matching actual client use.
• After the exposure period, each coupon is extracted for the surviving population using the qualified method.
• Disinfectants are used near their expiration date if possible.